MS Biotechnology - Track 1: DNA Technology

The Graduate Division of the FHS offers Master of Science (M.S.) degrees. The main areas of instruction are in Biotechnology, Bio-analytical Toxicology and Optics and Optometry. The Biotechnology program has two tracks: DNA Technologies and Forensic Science. Offered courses include basic biology and chemistry to advanced clinical molecular genetics band Forensics.

The Ministry of Higher Education authorized the American University of Science and Technology to establish the Faculty of Health Sciences (FHS) in accordance with the file presented to the Council of Higher Education as per decree number 677 dated August 30, 2007. This was later followed by the permit to start teaching in the majors of Clinical Laboratory Science (CLS) and Optics and Optometry (OAO).

 

 
 
TOTAL REQUIRED CREDITS FOR GRADUATION (36 credits)
CORE REQUIREMENTS (12 credits)
CODE DESCRIPTION PREREQUISITE CR.
CLS 522 Cell & Molecular Biology NONE
3
CLS 524 Current Genetic Analysis NONE
3
CLS532L Basic Molecular Biology Tools Pre OR Co. CLS 522
3
CLS534L Current Techniques in Molecular Biology CLS 522 & CLS532L
3
GRADUATION REQUIREMENTS (6 credits)
CODE DESCRIPTION PREREQUISITE CR.
CLS 598 Master's Thesis II Approval of Advisor
3
CLS 599 Master's Thesis Approval of Advisor
6
MAJOR REQUIREMENTS: (18 credits)
CODE DESCRIPTION PREREQUISITE CR.
CLS 526 Recombinant DNA CLS 522 & CLS 524
3
CLS 560 Web Based Molecular Biology CLS 522 & CLS 524 & CLS532L
1
CLS 565 Biostatistics and Epidemiology NONE
3
CLS 570 Bioorganic Chemistry NONE
3
CLS 580 Seminars in Biotechnology NONE
1
CLS 590 Writing a Research Proposal Approval of Advisor
1
CLS 594 Clinical Trials NONE
3
CLS538L Modern Molecular Biology Applications CLS 524 & CLS534L
3
CLS539L Research Methods in Molecular Biology CLS538L
3
Course Descriptions
CLS 522
Cell & Molecular Biology
This course will cover selected topics in the organization and regulation of the eukaryotic cell with an emphasis on cell structure and function, transcription regulation, RNA processing, nuclear transport, cell cycle control, as well as recombinant DNA methods and tools.
Prerequisite: NONE
CLS 524
Current Genetic Analysis
This course deals with fundamental and advanced concepts in the field of genetics. It emphasizes mainly genes and genomes, gene inheritance, genes and chromosome mutations, control of development as well as population and evolutionary genetics.
Prerequisite: NONE
CLS532L
Basic Molecular Biology Tools
This course is a comprehensive overview of key techniques in molecular biology related to DNA, RNA and proteins. The course covers the techniques of DNA, RNA and protein extraction, gel electrophoresis, PCR, restriction digestion, RT-PCR and protein SDS-PAGE.
Prerequisite: Pre OR Co. CLS 522
CLS534L
Current Techniques in Molecular Biology
This laboratory course is designed to familiarize students with current techniques in molecular biology. It encompasses hallmark applications providing students with practical knowledge of DNA nucleic acids and protein blotting and detection. In addition, students will acquire skills in clinically oriented applications such as ELISA, branched-PCR and RFLP.
Prerequisite: CLS 522 & CLS532L
CLS 598
Master's Thesis II
Each graduate student is required to submit, under the supervision of a faculty member, a research proposal in a grant application form following NIH guidelines in related field of study. Students will present and defend their proposals.
Prerequisite: Approval of Advisor
CLS 599
Master's Thesis
Students conduct laboratory research under the guidance of a faculty member in the faculty of Health Sciences in a selected area. Students should submit a written report and present and defend their projects.
Prerequisite: Approval of Advisor
CLS 526
Recombinant DNA
CLS 526 is an advanced course in molecular biology. Its objective is to offer to the student an extensive knowledge of the principles and practices of applying microbial agents in molecular biology research. The use of plasmids, phages, viruses, bacteria and yeast to study molecular structure and function of genes are emphasized.
Prerequisite: CLS 522 & CLS 524
CLS 560
Web Based Molecular Biology
This course visualizes biology in terms of computer science and presents the most significant algorithms of computational biology. The student will learn to process molecular biology data related to RNA, DNA and proteins with emphasis on blasting, aligning, and analyzing DNA sequences, as well as determining protein secondary and tertiary structures.
Prerequisite: CLS 522 & CLS 524 & CLS532L
CLS 565
Biostatistics and Epidemiology

This is advanced course in the applications of biostatistics and epidemiology into research projects. The goal is to present latest research statistical methodology useful in analyzing biomedical data. The course covers both theory and applications in the form of problem solving and applications on current research projects in Biotechnology and Bio-analytical toxicology.

Prerequisite: NONE
CLS 570
Bioorganic Chemistry
This is an advanced course designed to provide biochemical principles and applications pertinent to cell function and membrane structure. Research-related biochemical methodologies of macromolecules identification and analysis will be emphasized.
Prerequisite: NONE
CLS 580
Seminars in Biotechnology
Includes review and scientific critique of literature, overview of research design and style of scientific papers related to Biotechnology. Students will present papers related to current literature on a weekly basis. In addition, students will attend a minimum of seven seminars by visiting scientists and submit a final report listing the date, title and name of the speaker along with a one paragraph summary of each seminar.
Prerequisite: NONE
CLS 590
Writing a Research Proposal
The student selects a project of choice from a list of projects determined by a faculty member in a selected area of interest. The student should plan, write a proposal and submit a written report, present and defend the proposal. Laboratory experiments are not required.
Prerequisite: Approval of Advisor
CLS 594
Clinical Trials

This course is intended to provide an overview of the objectives, research strategies and methods of patient-oriented research. The course topics include competing objectives of clinical research, principles of observational studies, principles of clinical trials, principles of meta-analysis, interpretation of diagnostic tests, prognostic studies, causal inference, qualitative research methods, and decision analysis. Sessions generally combine a lecture on the topic with discussion of articles that are distributed in advance of the sessions.

Prerequisite: NONE
CLS538L
Modern Molecular Biology Applications
This course is designed to familiarize students with mastering skills in modern techniques in molecular biology including quantitative real-time PCR, mitochondrial DNA extraction and sequencing and STR amplification and detection. These are conventional techniques used DNA quantitation, diagnostic molecular biology, nucleic acid typing, forensic DNA profiling and paternity testing.
Prerequisite: CLS 524 & CLS534L
CLS539L
Research Methods in Molecular Biology
This course is designed to equip students with routine molecular biology techniques applied in basic research laboratories. The course aims at providing students with bench-work skills in subcloning and expression of genes; immunoprecipitation, immunoblotting and detection of cellular proteins; In-vitro coupled transcription and translation; specialized PCR and dot-blotting; microarray technologies and mutation detection by denaturing high pressure liquid chromatography (DHPLC).
Prerequisite: CLS538L

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